I know of a couple friends who plans to head down to the Apple store after work, but I don't know of any friends/colleagues who are currently standing in line.
I have not checked (in fact I have not been outside today), but I would guess that the lines around here (somewhere between Cincinnati and Dayton, Ohio) are not nearly as long as the lines in NYC, Boston, San Francisco, Chicago, Atlanta, etc. I wouldn't be surprised to drive by the local AT&T/Cingular store and see no line at all. I might have to think of something that I need from the mall just so I can drive by and check.
I'm anxious to see whether these things will really change the game or if they're just another gadget.