After updating to ASP.NET AJAX 1.0 you may get the following Javascript Error (especially for pages making use of animation):
“Sys.Debug.IsDebug is null or not an object”
or something else about Sys.Debug, depending on your browser of choice.
The issue is that the Javascript files being returned by IIS are cached copies from the previous release and are no longer valid with the code from the new release.
The fix is to run iisreset (or manually stop and restart IIS).
UPDATE: My cohort, Kyle Beyer, has put together a nice summary of the breaking changes for ASP.NET AJAX 1.0 RTM.